FIGURE 5.

BTK inhibition impairs NLC–mediated response against A. fumigatus and zymosan. NLC were treated overnight with ibrutinib or acalabrutinib and then stimulated with A. fumigatus conidia for 2 h or with zymosan for 1 h. (A) Blots show the signaling pathways affected by ibrutinib during A. fumigatus stimulation. Ibrutinib efficiently inhibited phosphorylation of BTK, STAT1 and IκBα during conidia stimulation (n = 4). (B) Ibrutinib affects phosphorylation of AKT and IκBα during zymosan stimulation (n = 4). (C) NLC were treated with acalabrutinib overnight and then stimulated with zymosan for 1 h. Blots show a reduction of IκBα and AKT either in presence or absence of zymosan stimulation (n = 4).