KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-GFP | Roche | 11814460001 |
| Rabbit polyclonal anti-VDAC | Cell Signaling Technology | 4866 |
| Rabbit polyclonal anti-Larp | Blagden et al., 2009 | https://doi.org/10.1016/j.ydbio.2009.07.016 |
| Rabbit polyclonal anti-MDI | Zhang et al., 2016 | https://doi.org/10.15252/embj.201592994 |
| Mouse monoclonal anti-actin, clone C4 | Millipore | MAB1501 |
| Goat anti-Biotin, HRP-linked | Cell Signaling Technology | 7075 |
| Rabbit polyclonal anti-Tom20 | Cell Signaling Technology | 13929 |
| Rabbit polyclonal anti-Phospho - (Ser/Thr) | Abcam | ab117253 |
| Rabbit polyclonal anti-Tamas | Zhang et al., 2015 | https://doi.org/10.15252/embr.201439636 |
| Rabbit polyclonal anti-mCherry | Biovision | 5993-100 |
| Chemicals and competent cells | ||
| TMRM | Thermo Fisher Scientific | I34361 |
| MitoTracker™ Green | Thermo Fisher Scientific | M7514 |
| Xho1 | NEB | R0146 |
| Click-IT™ AHA (L-Azidohomoalanine) | Thermo Fisher Scientific | C10102 |
| Biotin Alkyne | Thermo Fisher Scientific | B10185 |
| Glutathione Sepharose 4B | GE Life sciences | 17075601 |
| One Shot™ BL21(DE3) Chemically Competent E. coli | Thermo Fisher Scientific | C600003 |
| TMTsixplex™ Isobaric Label Reagent Set | Thermo Fisher Scientific | 90061 |
| Phosphatase Inhibitor Cocktail 2 | Sigma-Aldrich | P5726 |
| Phosphatase Inhibitor Cocktail 3 | Sigma-Aldrich | P0044 |
| ATP | Cell Signaling Technology | 9804 |
| Kinase Buffer (10X) | Cell Signaling Technology | 9802 |
| Grace's Insect Medium, Unsupplemented (methionine-free medium) | Thermo Fisher Scientific | 11595030 |
| Drosophila Gateway Vector Collection | DGRC | N/A |
| Critical Commercial Assays | ||
| Click-iT™ Plus EdU Alexa Fluor™ 555 Imaging Kit | Thermo Fisher Scientific | C10338 |
| Click-iT™ Protein Reaction Buffer Kit | Thermo Fisher Scientific | C10276 |
| GeneJET Gel Extraction Kit | Thermo Fisher Scientific | K0692 |
| QIAamp DNA Blood Mini Kit | Qiagen | 51106 |
| In-Fusion® HD EcoDry™ Cloning Plus | Takarabio | 638915 |
| DNA 7500 Kit | Agilent | 5067-1506 |
| μMACS GFP Starting Kit | Miltenyi Biotec | 130-091-288 |
| Experimental Models: D. melanogaster | ||
| w1118 (mt: wt) | Bloomington Drosophila Stock Center | N/A |
| w1118 (mt: ts) | Hill et al., 2014 | https://doi.org/10.1038/ng.2920 |
| pink1-gfp | This study | N/A |
| Tom20-mcherry | Zhang et al., 2016 | https://doi.org/10.15252/embj.201592994 |
| pink1-gfp;Tom20-mcherry | This study | N/A |
| pink1B9 | Clark et al., 2006 | https://doi.org/10.1038/nature04779 |
| mdi1 | Zhang et al., 2016 | https://doi.org/10.15252/embj.201592994 |
| UASp-Tom20-PINK1 | This study | N/A |
| larp-gfp | This study | N/A |
| mdi-gfp | Zhang et al., 2016 | https://doi.org/10.15252/embj.201592994 |
| UASp-Tom20-larp | This study | N/A |
| UASp-Tom20-larpS66D,T107E | This study | N/A |
| UASp-Tom20-larpS66A,T107A | This study | N/A |
| UASp-Tom20-larpS182D,S186D,S219D,S221D | This study | N/A |
| UASp-Tom20-larpS182A,S186A,S219A,S221A | This study | N/A |
| UASp-Tom20-larp T828E | This study | N/A |
| UASp-Tom20-larpT828A | This study | N/A |
| UASp-Tom20-larpS1119D | This study | N/A |
| UASp-Tom20-larpS1119A | This study | N/A |
| UASp-Tom20-larpS1510D,S1512D | This study | N/A |
| UASp-Tom20-larp S1510A,S1512A | This study | N/A |
| UASp-mCherry-ATG8 | Wang et al., 2016 | https://doi.org/10.1093/hmg/ddw067 |
| UASp-mito-GFP | Hill et al., 2014 | https://doi.org/10.1038/ng.2920 |
| Oligonucleotides | ||
|
pink1 chiRNA targeting sequence: 5’GTCAATAATTATTGTACCGG3’ |
This study | N/A |
|
pink1, genotyping Forward: 5’GATCACAGTCTTCGCGGCCT3’ |
This study | N/A |
|
pink1, genotyping Reverse 5’TTACTTGTACAGCTCGTCCATG3’ |
This study | N/A |
|
larp chiRNA targeting sequence: 5’GATTGGATTGATATAGACTG3’ |
This study | N/A |
|
larp, genotyping Forward: 5’GAAGTTCTGGGCCTTCCTGAAA3’ |
This study | N/A |
|
larp, genotyping Reverse: 5’TTACTTGTACAGCTCGTCCATG3’ |
This study | N/A |
|
mt:Co1, Xho1 site genotyping Forward: 5’TGGAGCTATTGGAGGACTAAATCA3’ |
Hill et al., 2014 | https://doi.org/10.1038/ng.2920 |
|
mt:Co1, Xho1 site genotyping Reverse: 5’GCTCCTGTTAATGGTCATGGACT3’ |
Hill et al., 2014 | https://doi.org/10.1038/ng.2920 |
| QPCR, his4, Forward: 5’TCCAAGGTATCACGAAGCC3’ |
Zhang et al., 2015 | https://doi.org/10.15252/embr.201439636 |
| QPCR, his4, Reverse: 5’AACCTTCAGAACGCCAC3’ |
Zhang et al., 2015 | https://doi.org/10.15252/embr.201439636 |
| QPCR, mt:Co1, Forward: 5’ATTGGAGTTAATTTAACATTTTTTCCTCA3’ |
Zhang et al., 2015 | https://doi.org/10.15252/embr.201439636 |
| QPCR, mt:Co1, Reverse: 5’AGTTGATACAATATTTCATGTTGTGTAAG3’ |
Zhang et al., 2015 | https://doi.org/10.15252/embr.201439636 |
| Recombinant DNA | ||
| PPWG-Tom20-PINK1 | This study | N/A |
| pGEX Larp | This study | N/A |
| pGEX PINK1 | This study | N/A |
| pGEX PINK1 KD | This study | N/A |
| pGEX Larp10A | This study | N/A |
| attB-UASp LarpS66D,T107E | This study | N/A |
| attB-UASp LarpS66A,T107A | This study | N/A |
| attB-UASp LarpS182D,S186D,S219D,S221D | This study | N/A |
| attB-UASp LarpS182A,S186A,S219A,S221A | This study | N/A |
| attB-UASp LarpT828E | This study | N/A |
| attB-UASp LarpT828A | This study | N/A |
| attB-UASp LarpS1119D | This study | N/A |
| attB-UASp LarpS1119A | This study | N/A |
| attB-UASp LarpS1510D,S1512D | This study | N/A |
| attB-UASp LarpS1510A,S1512A | This study | N/A |
| Software and Algorithms | ||
| GraphPad Prism7 | GraphPad Software, Inc. | http://www.graphpad.com/ |
| ImageJ2 | NIH | https://imagej.nih.gov/ij/ |
| Scaffold 4.0 | Proteome Software, Inc | http://www.proteomesoftware.com |
| Volocity | Quorum Technology | https://www.quorumtechnologies.com |
| Deposited Data | ||
| doi:10.17632/9vyphxf47r.1 | ||