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. 2020 Aug 28;11:1868. doi: 10.3389/fimmu.2020.01868

Figure 6.

Figure 6

IL-2/IL-15-expanded Vγ9Vδ2 T cells retain increased cytotoxicity against FM-28/ZOL under hypoxic conditions. FM-28 cells were cultured in hypoxia for 5 days prior to seeding in the xCELLigence and stimulated with 10 μM ZOL for the last 24 h. 25-day-expanded Vγ9Vδ2 T cells were added at an effector–target ratio of 1:1 and cytolysis monitored for 72 h in 1% O2. Cytolysis plots of donors (A) γδHD2, (B) γδHD10, and (C) γδHD12 comparing cytotoxicity against FM-28/ZOL between expansion conditions in 1% O2. Black, round symbols mark IL-2-expanded cultures, while red, square symbols mark IL-2/IL-15-expanded cultures. (D) Time to kill 50% of target cells FM-28/ZOL (KT50) is depicted for IL-2-expanded and IL-2/IL-15-expanded Vγ9Vδ2 T cell at an effector–target ratio 1:1 (n = 3 in one experiment; γδHD2,10,12). % cytolysis and KT50 were calculated with the xCELLigence RTCA Software Pro.