Table 1.
The design, construction, and features of some major company-owned antibody-phage libraries.
| Library | Type | Source | Size | Note | References |
|---|---|---|---|---|---|
| CAT-BMV | Naïve scFv | PBL, tonsils | 1.4 × 1010 | A total of 43 healthy donors | (173) |
| CAT-CS | Naïve scFv | Spleen, fetal liver | 1.29 × 1011 | cDNA from 160 donors | (174) |
| CAT-BMV | Naïve scFv | Spleen | 1.2 × 1011 | Derived from the germline VH gene DP47 from CAT-BMV library, and B-cell-derived VL and CDRH3 from CAT-CS library | (175) |
| Dyax | Naïve Fab | PBL, spleen | 3.7 × 1010 | PBL from 4 healthy donors, and part of a tumor-free spleen removed from a patient with gastric carcinoma | (176) |
| Morphosys's HuCAL® | Synthetic Fab | — | 2.1 × 1009 | CDRH3 and CDRL3 were diversified by TRIM | (177) |
| Morphosys's HuCAL GOLD® | Synthetic Fab | — | 1.6 × 1010 | All CDRs were diversified by TRIM | (178) |
| Morphosys's HuCAL PLATINUM® | Synthetic Fab | — | 4.5 × 1010 | HuCAL PLATINUM® is an advanced version of HuCAL GOLD®. All CDRs were diversified by TRIM, with additional sequence optimization to enhance mammalian cells expression and avoid undesirable motifs | (179) |
CAT, Cambridge Antibody Technology human antibody phage display library; HuCAL, Human combinatorial antibody library; PBL, Bone marrow, peripheral blood lymphocytes; TRIM, trinucleotide-directed mutagenesis method.