Figure 1.

Staurosporine induces glutathione depletion concomitant with caspase-3 activation. (A) HeLa cells were exposed to 1 μM staurosporine for a 4-h time interval. The concentration of cellular glutathione was determined by an enzymatic recycling assay, as described under “Materials and Methods.” All results are expressed as mean ± standard error. (B) Western blot analysis of caspase-3 activation during a 4-h exposure to staurosporine. ^*p < 0.05 against untreated control (n = 3).