Figure 4.

Serum hormone levels in bArKO and tArKO mice. Serum E₂ (A and F), T (B and G), A4 (C and H), FSH (D and I), and LH (E and J) were measured in bArKO and tArKO mice, respectively. Serum levels from 8- to 26-week-old mice shown in panels A and F-H were measured by LC-MS/MS and serum levels from 12-week-old mice shown in panels B and C were measured by ELISA and in panels D, E, I, and J were measured by RIA. For bArKO mice, n = 13 for controls and n = 6 for bArKO mice in A, and n = 8-12 per group in B-E. For tArKO mice, n = 25 for Het controls and n = 16 for tArKO mice in F-G and n = 7-8 per group in I and J. Two-tailed Student t test, *P < 0.05, ***P < 0.001, ****P < 0.0001. Serum FSH (K) and LH (L) levels following steroid hormone replacement in castrated bArKO mice were measured by RIA. Castrated mice were treated with vehicle (Veh), E₂, T, or T plus E₂ (TE₂) for 5 weeks. Mouse serum was collected from 14-week-old mice. One-way or 2-way ANOVA with Tukey multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 5 mice per group.