Skip to main content
. Author manuscript; available in PMC: 2020 Sep 11.
Published in final edited form as: J Orthop Trauma. 2018 Jun;32(6):288–295. doi: 10.1097/BOT.0000000000001160

Fig 3.

Fig 3

Effects of binge alcohol on BMP-2 protein expression in fracture callus. (A) Representative western blot for BMP-2 protein levels in fracture callus tissue homogenate at post fracture day 14. Saline samples labeled (S) and alcohol samples labeled (A). To ensure the equal loading of protein, blot signal volumes were normalized against total protein concentrations. Western data was reported as a ratio of average signal volumes between the control and experimental groups. Alcohol causes a significant increase in BMP-2 expression at post fracture day 14 as seen in the western blot data (p=.042), as well as in the ELISA immune assay (p=.0055) (B) Statistical significance was present when p <0.05 as determined by independent sample T-tests and displayed with (*).