Immunosuppressive factors produced by K14HPV16/H2b myeloid cells are increased in lymph nodes together with myeloid cell growth factors and proteins associated with inflammation. A) Western blot analysis of whole lymph node protein extracts from K14HPV16/H2b or FVBN/H2b mice. B) Western blots of protein extracts from total CD11b+ myeloid cells or myeloid cell-depleted CD11b− cells isolated from the spleen of K14HPV16/H2b or FVBN/H2b mice. C) Flow cytometry analysis of ROS production by CD11b+ cells measured in-vitro with DCFDA (Groups: FVBN/H2b, K14HPV16/H2b, n=5; positive controls with PMA, n=4). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P<0.0001; n.s., not significant.