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. 2020 Aug 28;11:1975. doi: 10.3389/fimmu.2020.01975

Figure 4.

Figure 4

NEAT1 competitively binds miR-410-3p to regulate YY1 levels in HFLS-RA cells (A) Binding sites of miR-410-3p in NEAT1 was predicted by ENCORI. (B) Interaction between NEAT1 and miR-410-3p was analyzed by dual-luciferase reporter assays. (C,D) The relative expression of miR-410-3p in HFLS-RA cells transfected with the pcDNA-NEAT1 or siRNAs against NEAT1. (E–G) The relative mRNA and protein levels of YY1 in HFLS-RA cells transfected with the pcDNA-NEAT1 or pcDNA-NEAT1 and miR-410-3p mimics. (H–J) The relative mRNA and protein levels of YY1 in HFLS-RA cells transfected with siRNAs against NEAT1 or siRNAs and miR-410-3p inhibitor. All the experiments were independently performed in triplicates. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with the relative NC groups.