Figure 1.

Experimental designs used in the study. For validation of the preclinical model, ibuprofen, gabapentin or vehicle was administered (100 mg/kg, p.o.) on days 8, 9, and 10. Intravesical instillation of DMSO (50%), Ialuril^® or vehicle (500 µL/rat) was performed under gaseous anesthesia on day 7. Von Frey test was first performed at day 7 (before pharmacological treatment) to confirm presence of visceral pain, and then at day 10 to analyze compounds effect on CYP-induced chronic visceral pain. Bladder inflammation was also assessed at D10 after von Frey test.