Figure 6.

Validation of the model by curative oral treatment of clinically relevant compounds. Rats received three injections of CYP (40 mg/kg, i.p.) on days 0, 3, and 6. Rats were randomized and treated with three oral administrations of either ibuprofen (100 mg/kg) or gabapentin (100 mg/kg) or vehicle (10 ml/kg) on days 8, 9, and 10. (A) Nociceptive response was assessed by von Frey testing at day 7, once the cystitis was established but before treatment start (Before), and at day 10, 1 h after the last treatment (Post). Nociceptive score was expressed as the percentage of the maximal score for each von Frey force. (B) Bladder inflammation was assessed at day 10 by measuring bladder weight, wall thickness and edema scores. Data represent mean values ± SEM (n = 11 for CYP and n = 12 for Gabapentin and Ibuprofen). ^nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 (two-way RM ANOVA). (A) Two-way RM ANOVA; (B) Mann Whitney or unpaired t test).