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Effect of i.t. apelin-13 on gene expression in the L4/5 spinal cord of mice. mRNA expression levels of Aplnr (A), Grin2a (B), Grin2b (C) and CamK2d (D) were normalized with the housekeeping gene 36B4 mRNA expression using real-time qPCR. Data are expressed as means ± SEM. n = 6–9 per group. *p < 0.05, compared with vehicle control (i.d. NS + i.t. NS); #p < 0.05, compared with CFA group (i.d. CFA + i.t. NS).
