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Effect i.t. apelin-13 on the c-Fos, Creb1, Dr1 and Egr1 gene expression in mouse L4/5 spinal cord. mRNA expression levels of Fos (A), Creb1 (B), Dr1 (C) and Egr1 (D) were determined by real-time qPCR and normalized with the housekeeping gene 36B4 mRNA expression. Data are expressed as means ± SEM. *p < 0.05, compared with vehicle control (i.d. NS + i.t. NS); #p < 0.05, compared with CFA group (i.d. CFA + i.t. NS). n = 8 or 9 per group.
