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. 2020 Aug 28;11:1335. doi: 10.3389/fphar.2020.01335

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Copyright © 2020 Lv, Zhang, Zhou, Feng, Yang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of apelin-13 on Fos protein expression in L4/5 spinal cord in mice. Representative sections of the lumbar spinal cord showing Fos-positive cells in the vehicle control (i.d. NS + i.t. NS, A), CFA group (i.d. CFA + i.t. NS, B) and apelin-13-treated group (i.d. CFA + i.t. NS, C). (D) Objective to measure and evaluate the distribution of Fos-positive cells of the lumbar spinal cord in mice. (E) Quantification of the number of Fos-positive cells in the spinal cord L4/5 segments from mice. Data are expressed as means ± SEM. *p < 0.05, compared with vehicle control (i.d. NS + i.t. NS); ^#p < 0.05, compared with CFA group (i.d. NS + i.t. CFA). n = 6 per group. Scale bars = 100 μm.