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. 2020 Aug 28;11:532457. doi: 10.3389/fphar.2020.532457

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Copyright © 2020 Yu, Li, Gu and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histopathology and immunofluorescence analyses. (A) Histopathological (H&E) analyses and (B) necrotic areas in H&E-stained tumor sections from 4T1-xenografted mice following treatment with NS as the control, DOX+CDDP, or HA-DOX-CDDP. Red arrows indicated the necrotic area. (C) Immunohistochemical (PARP and survivin) analyses of tumor tissue sections following treatment with NS as the control, DOX +CDDP, or HA-DOX-CDDP. (D) Relative optical densities of tumor sections showing PARP immunofluorescence. (E) Relative optical densities of tumor sections showing survivin immunofluorescence. Data are presented as the mean ± SD (n = 5). *P < 0.05, **P < 0.01.