Figure 5.

SOE alleviated IL-1β–induced proliferation, migration, and invasion in the human synovial cell line. Cells were seeded in 96-well (1 × 10⁴ cells/well), 24-well (1 × 10⁵ cells/well) and 6-wells (5 × 10⁵ cells/well) plates and allowed to grow for 24 h. A scratch wound was built in each group with a 10-μl pipette tip in the 24-well plate. Cells were co-treated with the indicated concentrations of SOE for 24 h in the absence or presence of IL-1β (20 ng/ml). The co-treated cells were transferred into transwell plates to analyze the migration and invasion. The cytotoxicity of SOE to MH7A and SW982 cells (A). Inhibition of SOE on IL-1β–induced proliferation (B, C), migration (D, E) and invasion (F) in the human synovial cell line. Statistical data for scratch wound healing (G), migration (H) and invasion (I). (^#P < 0.05, ^###P < 0.001 vs. Ctrl group; ^*P < 0.05, ^***P < 0.01 vs. IL-1β group, n = 3).