Figure 7.
SOE reduced the expression of COX-2, NLRP3, MMP9, and the phosphorylation of MAPKs but increased the expression of MMP2 in IL-1β–induced SW982 cells. SW982 cells were seeded in 60 mm cell culture dish (1 × 106 cells/well) and allowed to grow for 24 h. Cells were pretreated with SOE (50, 100, 200 μg/ml) for 1 h, then stimulated with IL-1β (20 ng/ml) for 12 h or 1 h. The expression levels of COX-1, COX-2, NLRP3, MMP9, MMP2 (A) and MAPKs (B) were detected by Western blotting. (##P < 0.01 vs. Ctrl group; **P < 0.01 vs. IL-1β group, n = 3).