Table 1. Plasmids and strains used in this study.
Plasmids and strains ending in the letter “c” for “cytosolic” express heterologous PHB enzymes without localization tags. Plasmids and strains ending in “p” for “peroxisomal” express enzymes modified with enhanced Peroxisome Targeting Sequences (ePTS1).
| Plasmid | ||
| Name | Description | |
| pPHBc | pRS416-(adh1t-phaA-pgk1p)-(tef1p-phaB-cyc1t)-(pdc1p-phaC-pdc1t) | |
| pPHBp | pRS416-(adh1t-ePTS1-phaA-pgk1p)-(tef1p-phaB-ePTS1-cyc1t)-(pdc1p-phaC-ePTS1 pdc1t) | |
| Strains | ||
| Name | Phenotype | Plasmid |
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | |
| phbC | Same as BY4741 | pPHBc |
| phbP | Same as BY4741 | pPHBp |