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. 2020 Aug 28;11:1321. doi: 10.3389/fphar.2020.01321

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Copyright © 2020 Yu, Li, Wang, He, Liu, Ma, Pang, Jiang, Lu, Li and Qi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CTD induced apoptosis of HL-60 cells. HL-60 cells were treated with CTD as indicated for 48 h. (A, B) Apoptotic cells were determined by flow cytometry and Hoechst 33342 staining (n = 3). (C) HL-60 cells were pre-treated with the pan-caspase inhibitor Z-VAD-FMK for 2 h and then treated with CTD as indicated for 48 h. Cell viability was measured using CCK-8 assay. (D) HL-60 cells were treated with CTD as indicated for 48 h and then apoptosis-related proteins were detected by Western blotting. The blots were a representative of three independent experiments. The scale bar is 100 μm. Values are presented as the means ± SD. **p < 0.01, ***p < 0.01 vs control.