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. 2020 Aug 28;11:1321. doi: 10.3389/fphar.2020.01321

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Copyright © 2020 Yu, Li, Wang, He, Liu, Ma, Pang, Jiang, Lu, Li and Qi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CTD suppressed proliferation and induced cell cycle arrest in HL-60 cells. (A) HL-60 cells were treated with CTD as indicated for 120 h, and cell proliferation assay was performed by trypan blue exclusion. (B) HL-60 cells were treated with CTD as indicated for 48 h. After RNase A treatment and PI staining, cell cycle was determined by flow cytometry quantitatively (n = 3). (C) The cell cycle related proteins were detected by Western blotting after treatment with CTD as indicated for 48 h. The blots were a representative of three independent experiments. Values are presented as the means ± SD.