Table 3.
In vitro research | Explant | Basal medium | Method | Duration (weeks) | Shoot multiplication | Rooting in vitro | Acclimatisation | |||
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PGRs (μM) | Result (shoots/explant) | PGRs (μM) | Result | Survival % | Duration | |||||
Dheeranupattana et al. (2003) | Shoot tips | MS | DR | 4 | 2.68 NAA, 13.32 BA | 2.4 | n/a | Spontaneous | 100% | 2 weeks |
Mongkolchaipak et al. (2006) | Rhizome buds | MS | DR | 4–8 | 31.08 BA | 4.2 to 4.5 | 5.37 NAA | Increased | 98% | 4 weeks |
Prathanturarug et al. (2007) | Rhizome buds | MS | 2 Step DR | 16 | 35.52 BA or 4.54 TDZ | 8.8 ± 0.6 or 12.8 ± 0.8 | 0 or 2.69 NAA | Spontaneous (0 NAA) | n.a. | n.a. |
Alveno (2012) | Young shoots | MS, ½ MS | DR | 3–4 | 16.65 BA | 1.77 | n.a. | n.a. | n.a. | n.a. |
Zuraida et al. (2014) | Rhizome buds | MS | Callus Induction | 13–14 | 4.55 2,4-D, 1.07 NAA | 20% callus formation | n.a. | n.a. | n.a. | n.a. |
Note: MS: Murashige and Skoog basal medium; DR: Direct regeneration method; BA: N6-benzyladenine; NAA: Naphthaleneacetic acid; TDZ: Thidiazuron; n/a: Non applicable