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. 2020 Apr 7;31(1):123–139. doi: 10.21315/tlsr2020.31.1.8

Table 3.

Summary of previous In vitro reports on Kaempferia parviflora.

In vitro research Explant Basal medium Method Duration (weeks) Shoot multiplication Rooting in vitro Acclimatisation



PGRs (μM) Result (shoots/explant) PGRs (μM) Result Survival % Duration
Dheeranupattana et al. (2003) Shoot tips MS DR 4 2.68 NAA, 13.32 BA 2.4 n/a Spontaneous 100% 2 weeks
Mongkolchaipak et al. (2006) Rhizome buds MS DR 4–8 31.08 BA 4.2 to 4.5 5.37 NAA Increased 98% 4 weeks
Prathanturarug et al. (2007) Rhizome buds MS 2 Step DR 16 35.52 BA or 4.54 TDZ 8.8 ± 0.6 or 12.8 ± 0.8 0 or 2.69 NAA Spontaneous (0 NAA) n.a. n.a.
Alveno (2012) Young shoots MS, ½ MS DR 3–4 16.65 BA 1.77 n.a. n.a. n.a. n.a.
Zuraida et al. (2014) Rhizome buds MS Callus Induction 13–14 4.55 2,4-D, 1.07 NAA 20% callus formation n.a. n.a. n.a. n.a.

Note: MS: Murashige and Skoog basal medium; DR: Direct regeneration method; BA: N6-benzyladenine; NAA: Naphthaleneacetic acid; TDZ: Thidiazuron; n/a: Non applicable