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. Author manuscript; available in PMC: 2020 Sep 11.
Published in final edited form as: J Am Chem Soc. 2020 Jun 9;142(25):11032–11041. doi: 10.1021/jacs.0c02796

Figure 2.

Figure 2.

Surrounding residues of the GFP chromophore in the protonated state (A state) for avGFP, whose residue 65 is a serine.18 The ESPT chain is explicitly shown here. The colored residues represent those mutated in this work. Note that E222 mutants cannot be used for investigating the A state as the mutation suppresses chromophore protonation.13