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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Anal Chem. 2019 Sep 12;91(19):12560–12567. doi: 10.1021/acs.analchem.9b03491

Figure 1.

Figure 1.

Sequence coverage map of the CaM protein sequence. Black lines under the sequence indicate tryptic peptides whereas red lines designate chymotryptic peptides. The four EF-hands are super-scored by red (EF-1), magenta (EF-2), olive (EF-3) and navy (EF-4). Residues shaded in green, cyan and dark yellow represent the E helix, calcium loop and F helix of each EF-hand, respectively. Residues boxed in red are resolved by MS/MS analysis.