FIG 4.

qPCR-based assessment of D. melanogaster microbiota. (A to F) Molecular quantification of total bacteria, bacterial genera, and species in whole-body D. melanogaster adults. (E) Intraindividual Lactobacillus/Acetobacter load ratios. All comparisons were made after normalizing to total host DNA. Data are depicted as means ± standard deviations. Significance was determined with Tukey’s multiple-comparison tests. Each point represents a single D. melanogaster adult from a separate experimental vial (n = 10; three technical replicates were performed). Sexes are pooled. *, P < 0.05; ***, P < 0.001; ns, not significant.