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. 2020 Aug 28;12(16):16304–16325. doi: 10.18632/aging.103660

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Copyright © 2020 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HOXD-AS1 promoted stemness maintenance and chemo-resistance in CCA. (A) Spheroid forming abilities of transfected QBC939 and CCLP-1 cells were evaluated by spheroid formation assay. (B) The effect of HOXD-AS1 on stem markers (SOX2, OCT4, Nanog) was detected by western blot. (C) The effect of HOXD-AS1 on the treatment of cisplatin and gemcitabine was measured by CCK-8 assay. (D) Mice were subcutaneously injected with QBC939 cells transfected with HOXD-AS1 plasmid and intraperitoneally injected with PBS, cisplatin or gemcitabine. (E) The stem markers (SOX2, OCT4, Nanog) in xenograft tumors were measured by western blot. ^*P < 0.05, ^**P < 0.01.