Figure 4.

Mitochondrial biogenesis is increased by SirT3 via the AMPK pathway. (A–C) RNA was isolated from cardiomyocytes, and then transcription of mitochondrial biogenesis parameters was determined. Lentivirus-loaded SirT3 (SirT3-OE) and AMPK agonist (AICAR) were incubated with cardiomyocyte before LPS treatment. Compound C (CC), an antagonist of AMPK, was used to inhibit the activation of AMPK in SirT3-OE–transfected cardiomyocytes. (D–F) Immunofluorescence assay for Nrf2 and PGC1α in the presence of LPS. *P < 0.05.