Fig 1.

a: Liver and spleen attenuation measurements. Attenuation (in HU) of liver and spleen was measured using three regions of interest, each at least 2 cm² in area to ensure measurements are representative of the organ. A difference between mean spleen and liver attenuations of at least 10 HU was used to determine presence of hepatic steatosis. b: Skeletal muscle cross-sectional area at L3 level. Skeletal muscle at the L3 level was outlined (green line). Then, an attenuation threshold between -29HU and +150HU was applied to refine the region of interest on a pixel-wise basis to determine the cross-sectional area of muscle (red). c: Subcutaneous fat measurement at L3. For subcutaneous fat, the abdominal cavity was outlined (green line), An attenuation threshold of -30HU or less was applied outside the abdominal cavity to refine the region of interest on a pixel-wise basis to determine the cross-sectional area of the subcutaneous fat (red). d: Visceral muscle -sectional area at L3. For visceral fat, the abdominal cavity was outlined (green line) and an attenuation threshold of -30HU or less was applied inside the abdominal cavity to refine the region of interest on a pixel-wise basis to determine the cross-sectional area of visceral fat (red).