Table 1. List of primary and secondary antibodies with corresponding dilutions used in the current study.
Antibodies | Species | Company/Cat. # | Dilution (DRG & V1) |
---|---|---|---|
Anti-CD68 | Rat | Serotec/MCA1957 | 1:2000 |
Anti-GFAP | Rabbit | Dako/Z0334 | 1:5000 |
Anti-GFAP | Chicken | Abcam/ab4674 | 1:1000 |
Anti-GFP | Chicken | Invitrogen/A10262 | 1:1000 |
Anti-GLAST | Rabbit | Frontier Institute/Af660 | 1:5000 |
Anti-Iba1 | Rabbit | Wako/019-1974 | 1:2500 (DRG) |
1:1000 (V1) | |||
Anti-NeuN | Guinea Pig | Millipore/ABN90 | 1:1000 |
Anti-NF | Rabbit | Millipore/AB9568 | 1:1000 |
Anti-S100β | Rabbit | Abcam/ab52642 | 1:1000 |
Alexa Fluor® 488 anti-chicken | Goat | Invitrogen/A-11039 | 1:1000 |
Alexa Fluor® 546 anti-guinea pig | Goat | Invitrogen/A-11074 | 1:1000 |
Alexa Fluor® 546 anti-rabbit | Goat | Invitrogen/A-11035 | 1:1000 |
Alexa Fluor® 546 anti-rat | Goat | Invitrogen/A-11081 | 1:1000 |