Table 2. Data quantification in DRG SGCs and V1 astrocytes from the mouse lines used in the current study.
DORSAL ROOT GANGLIA | |||||||
---|---|---|---|---|---|---|---|
Mouse lines | Nbr of mice, Nbr of slices/mouse | Total NeuN+ or NF+ neurons | Total GFP+ or G6+ neurons | % of GFP+ or G6+ neurons | Total GLAST+SGCs | Total GFP+ or G6+ SGCs | % of GFP+ or G6+ SGCs |
S100β-eGFP | 3, 10 | 599 | 81 | 13.5 | 345 | 296 | 85.8 |
ALDH1L1-eGFP | 3, 11 | 1002 | 72 | 7.2 | 571 | 322 | 56.4 |
GFAP-Cre::GCaMP6f | 3, 14 | 1189 | 696 | 58.5 | 1250 | 22 | 1.8 |
GLAST-CreERT2::GCaMP6f | 4, 15 | 1148 | 44 | 3.8 | 892 | 48 | 5.4 |
Cx30-CreERT2::GCaMP6f | 3, 8 | 837 | 0 | 0 | 721 | 0 | 0 |
Cx43-CreERT2::GCaMP6f | 3, 9 | 433 | 31 | 4.0 | 363 | 336 | 92.6 |
VISUAL CORTEX | |||||||
Mouse lines | Nbr of mice, Nbr of slices/mouse | Total NeuN+ neurons | Total GFP+ or G6+ neurons | % of GFP+ or G6+ neurons | Total S100β+astrocytes | Total GFP+ or G6+ astrocytes | % of GFP+ or G6+ astrocytes |
S100β-eGFP | 3, 10 | 4737 | 1 | 0.02 | 1288 | 1108 | 86.0 |
ALDH1L1-eGFP | 3, 8 | 7807 | 0 | 0 | 1360 | 1117 | 82.1 |
GLAST-CreERT2::GCaMP6f | 3, 9 | 9533 | 3 | 0.03 | 1267 | 713 | 56.3 |
Cx30-CreERT2::GCaMP6f | 3, 9 | 5343 | 5 | 0.1 | 1000 | 464 | 46.4 |
Cx43-CreERT2::GCaMP6f | 3, 9 | 5203 | 227 | 4.4 | 780 | 74 | 9.5 |
Abbreviations: G6, GCaMP6f; GFP, green fluorescent protein; Nbr, number; NF, neurofilament.