Table 4.

Relevant fields to document for an in vitro assay. Example: bacterial mutation assay

Compound identifier:	CASRN: 462–06-6; Fluorobenzene; Benzene, fluoro-
Compound purity:	Not reported
Compound solubility:	Not reported
Solvent	DMSO
Study call:	Positive
Title:	Genetic Toxicity Evaluation of Fluorobenzene in Salmonella/E.coli Mutagenicity Test or Ames Test. Study 639736
Reference:	https://tools.niehs.nih.gov/cebs3/ntpViews/?activeTab=summary&studyNumber=639736
Study type:	Bacterial mutagenicity (Ames)
Source:	National Toxicology Program
Species:	Salmonella typhimurium
Strain/Cell type (Number of tests):	TA98 (N=6); TA100 (N=7); TA1535 (N=2)
Metabolic activation (Number of tests):	Absent (N=4); Present (N=11)
Metabolic activation system:	Aroclor 1254 treated rat liver S-9 fraction (30%), Aroclor 1254 induced hamster liver S-9 fraction (30%)
Dose summary:	0 – 1666 µg/plate; 0 – 750 µg/plate
Toxicity:	No cytotoxicity reported
Method:	Pre-incubation; Plate test – vapor from liquid
Controls used: Strain (wo S9/w S9):	Positive controls: TA98 (4-Nitro-o-phenylenediamine/2-aminoanthracene), TA100 (Sodium azide/2-aminoanthracene), TA1535 (Sodium azide/2-aminoanthracene)
Control values within historical ranges:	Yes
OECD test guideline:	471
Current guideline compliance	No, insufficient bacterial tester strains used
Study Report:	https://tools.niehs.nih.gov/cebs3/ntpViews/?activeTab=detail&studyNumber=639736&reportFormat=XLS
GLP compliance:	No
Year conducted	1991
Klimisch score:	3
Rationale for reliability incl. deficiencies:	Not tested up to guideline recommended concentrations and insufficient number of strains and not tested according to GLP