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. 2020 Aug 31;16(8):e1008780. doi: 10.1371/journal.ppat.1008780

Fig 6. OsUBL5.1 mediates p3 degradation through tbe 26S proteasome system and interacts with OsRPN10 and OsRPN13 from O. sativa.

Fig 6

A. Myc-fused OsUBL5 had no effect on GFP expression on leaves when it was co-expressed with GFP by agroinfiltration. B. Myc-fused OsUBL5 reduced GFP-fused p3 protein accumulation when they were co-expressed. C. Reduction of GFP-fused p3 protein caused by Myc-fused OsUBL5 was inhibited by MG132 treatment. Myc-fused pGus2 was used as control. GFP and Myc-fused proteins were detected by western blot. D and E. Co-IP showed the interaction of OsUBL5 with OsRPN13 and OsRPN10, respectively. Myc-fused pGus3 was used as control. Results are from three biological replicates. Band intensity in blots was calculated by ImageJ based on three replicates. A two-sample unequal variance directional t test was used to test the significance of the difference (**, p value<0.01).