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. 2020 Aug 31;16(8):e1008822. doi: 10.1371/journal.ppat.1008822

Fig 5. The exocyst is involved in facilitating the efficient cytosolic escape of S. flexneri.

Fig 5

(A) Illustration of the study of the effect of the exocyst on S. flexneri invasion in relation to the time of disassembly of S. flexneri BCV (black rectangle) and the time of formation of actin tails (orange rectangle) in different conditions including (B-C) RNA interference of non-targeting control (siRNA Neg) versus Sec5 depletion (siRNA Sec5), (D-E) RNA interference of non-targeting control (siRNA Neg) versus Exo70 depletion (siRNA Exo70), (F-G) expression of wild-type Sec5 versus cells expressing Sec5 lacking the core exocyst assembly motif (Sec5-ΔCorEx) and (H-I) expression of wild-type Exo70 versus cells expressing Exo70 lacking the core exocyst assembly motif (Exo70-ΔCorEx). Individual BCVs and actin tails were analyzed in triplicates for each condition (n > 45). The bars (magenta) represent the mean and unpaired t-tests were carried out (*p<0.05; **p<0.01; ****p<0.0001).