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. 2020 Aug 31;16(8):e1008822. doi: 10.1371/journal.ppat.1008822

Fig 7. Schematic illustration of the role of the exocyst on the efficient cytosolic escape of S. flexneri.

Fig 7

(A) Recruitment of Sec5 (triangle, grey) and Exo70 (star, orange) by Rab8A (square, yellow) and Rab11A (square, light green). Sec5 is present on the S. flexneri IAMs and BCV, whereas Exo70 is exclusively present on the IAMs. (B) Successive events after the BCV rupture of S. flexneri, in the exocyst-assisted or exocyst-disrupted conditions, are depicted. The exocyst assists in organizing IAMs in close proximity to the rupturing BCV, facilitating the BCV disintegration that leads to a high proportion of S. flexneri moving free of any attached BCV membrane remnants. In contrast, IAMs are more dispersed in exocyst-disrupted conditions (e.g. depletion of the exocyst subunits by siRNA or expression of mutants that interrupt the assembly of the exocyst complex, as demonstrated by Sec5-ΔCorEx and Exo70-ΔCorEx in this study, or in the presence of the specific inhibitor of Exo70). S. flexneri is observed to stay longer within the damaged BCV or moves with the attachment of the perforated BCV in these conditions.