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. 2020 Aug 31;16(8):e1008801. doi: 10.1371/journal.ppat.1008801

Fig 9. RSV infection suppresses the BR biosynthesis and signal pathway.

Fig 9

(A) The relative expression levels of BR biosynthetic genes in non-infected and RSV-infected plants of the susceptible rice cultivar Suyunuo (SYN). (B) Levels of endogenous CS and 6-deoxoCS in RSV-infected and non-infected SYN rice plants. (C) Quantitative RT-PCR analysis showing the relative expression levels of BR signaling-related genes in RSV-infected and non-infected SYN rice plants. (D) Cell-free degradation assay of GST-OsGSK2 incubated with extracts from RSV-infected and non-infected SYN rice plants. OsHSP was used as loading control. The number on the panel indicate the protein level of GST-OsGSK2 relative to its initial value (0 h). The experiment was repeated three times. (E) Half-life plot for cell-free degradation of GST-OsGSK2 in (D). Data are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01 by the Student’s t-test in A, B, C and E.