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. 2020 Sep 11;6(37):eabc0367. doi: 10.1126/sciadv.abc0367

Fig. 2. LSD1 is required for crypt maturation in vivo and Wnt dependency of organoids.

Fig. 2

(A) Representative images of antibody (lysozyme and SOX9) and in situ hybridization (ISH) (Atoh1 and Wnt3) staining of WT and KO small intestinal tissue. (B) Quantification of MUC2+ goblet and CHGA+ enteroendocrine cells in adult duodenum intestine. N = 4 mice; *P < 0.05. (C) Representative images of MUC2 and CHGA antibody staining at P0.5. (D) Quantifications of MUC2 (mouse protein) GCs throughout development. N ≥ 3 mice; mean ± SEM is shown. (E) Representative image of OLFM4 (mouse protein) antibody and UEA-1 staining of adult WT and KO tissue. (F to H) Bright-field images of WT and KO organoids with indicated treatments; wells from two different experiments (n = 2 mice) are quantified. (I) Organoid outgrowth from single sorted Lgr5HIGH ISCs; each dot represents a mouse; data pooled from three independent experiments. Mean and SEM are shown.