Skip to main content
. 2020 Sep 11;6(37):eabc0367. doi: 10.1126/sciadv.abc0367

Fig. 4. LSD1 controls H3K4me1/2 levels of fetal-like genes.

Fig. 4

(A) Heatmaps of H3K4me1 and H3K4me2 sites that are significantly up in KO crypts compared to WT crypts. (B) Venn diagram comparing genes associated with H3K4me1 and H3K4me2 peaks that were significantly higher in KO crypts compared to WT crypts. (C) RNA-seq analysis by GSEA of KO versus WT transcriptional profile on a gene set consisting of genes with associated increased H3K4me1 levels in KO crypts. (D) GSEA of LSD1 core gene list (228 genes) on a transcriptome dataset that compares fetal to adult organoids (9). (E) Venn diagram of genes significantly higher expressed in EEDKO compared with genes that are up in P7vsP21 and E18vsP21. (F and G) Venn diagrams comparing the LSD1 core and the EED core (genes up in EED KO crypts AND have H3K27me3 associated peak) as well as the genes associated with increased methylation levels in each separate group.