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. 2020 Aug 11;9:e54572. doi: 10.7554/eLife.54572

Figure 1. Schematic of the RP2 and RP8 gene-break transposon (GBT) system with all three reading frames of AUG-less mRFP reporter.

(A–C) Schematic of the GBT system, RP2 and RP8 incorporate a protein-trap cassette fused with three reading frames of AUG-less mRFP reporter and a 3’ exon trap cassette with GFP or tagBFP reporters, respectively. (A) RP2 series (RP2.1, RP2.2 and RP2.3). Underline: Previously published vector construct (B–C) RP8 series (RP8.1, RP8.2 and RP8.3) with a schematic RP8 insertion event showing expected transcription off of a locus below (C). ITR: inverted terminal repeat, SA: splice acceptor, lox: Cre recombinase recognition sequence, *mRFP: AUG-less mRFP sequence, poly (A)+: polyadenylation signal, red octagon: extra transcriptional terminator and putative border element, β-act: carp beta-actin enhancer, γ-cry: gamma crystalline promoter, SD: splice donor, E: enhancer, P: promoter, and WT: wild-type.

Figure 1.

Figure 1—figure supplement 1. Representative expression patterns of mRFP fusion protein integrated all reading frames of RP2 and RP8.

Figure 1—figure supplement 1.

Lateral and dorsal views of representative bright field images at four dpf and lateral or dorsal views of RFP expression patterns at four dpf in GBT1577 integrated RP2.2, GBT1625 and GBT1629 integrated RP2.3, GBT0409 (npr2) and GBT0726 (radx) integrated RP8.1, GBT1599 integrated RP8.2 and GBT1631 integrated RP8.3. Scale bars = 200 µm.