Table 2.

Culture Conditions for the Four Methods Compared

B (blue) = improved 2D-multistep, C (pink) = 2D-simple, D (yellow) = 3D-multistep, E (orange) = 3D-simple. Cells were harvested at the time point of maximal CD34⁺ progenitor cell purity and/or colony-forming unit (CFU) capability for each method. Refer to Table 1 for explanation of + symbols and abbreviations. Additional abbreviations: IL3, interleukin-3; ITS, insulin transferrin-selenium; NHP, normal human plasma; O2, oxygen level; SLII, Stemline II hematopoietic stem cell expansion medium.