Table 3.

Cost and Relative Cost of Each Method to Produce the Same Number of Target Cells

Output	2D-Multistep	2D-Simple	3D-Multistep	3D-Simple
Per 106 CD34+ cells generated	$89 (relative cost = 1×)	$2,570 (29×)	$709 (8×)	$2,570 (29×)
Per 106 CFU generated	$5,230 (59×)	$14,182 (160×)	$69,848 (788×)	$10,637 (120×)

Calculations based on the 2018 list price and volume required for all reagents per well of culture, adjusted by the average numbers of CD34⁺ cells and total colony-forming units (CFU) produced per method to determine the required number of wells to produce 1 × 10⁶ of the target cells. Values in parentheses show cost relative to the 2D-multistep method for producing 1 × 10⁶ CD34⁺ cells stated as “relative cost = 1×,” which equated to US$88.64.