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. 2020 Aug 6;15(3):706–720. doi: 10.1016/j.stemcr.2020.07.005

Figure 2.

Figure 2

Hoxb6 Is a Target of miR-126-5p

(A) The 3′ UTR of Hoxb6 gene contains binding sites for miR-126-5p according to TargetScan (http://www.targetscan.org/). Black underlined sequences indicate the point mutations used to generate mouse Hoxb6 3′ UTR Mut constructs.

(B) Relative luciferase activity assays of luciferase reporter plasmid with mouse WT or Mut Hoxb6 3′ UTR, and that of vector were performed after co-transfection with miR-126-5p mimics or NC mimics in HeLa cells. Hoxb6 3′ UTR Mut seed complementary sites were shown in (A).

(C) HOXB6 protein levels were measured by western blotting after transient transfection with miR-126-5p mimics or NC into HIBEpic cells.

(D) Expression of HOXB6 was measured at the protein level in Ad.ctrl and Ad.sg126 mouse livers.

Data are expressed as means ± SD, n = 3 independent experiments containing three replicates. Significant difference is presented at the level of ∗∗p < 0.01 by two-tailed Student's t test. See also Figure S2.