Figure 3.

HOXB6 Negatively Regulates SOX9 Expression In Vitro and In Vivo

(A) HepG2 cells, HIBEpic cells, and LO2 cells were transfected with the HOXB6 siRNA (Si-HOXB6) or NC. At 48 h after transfection, western blotting and qRT-PCR analysis of HOXB6 and SOX9 expression in the NC group and the Si-HOXB6 group were performed.

(B) Western blotting and qRT-PCR analysis were conducted to detect HOXB6 and SOX9 expression in HepG2 and HIBEpic cells after HOXB6 exogenous overexpression.

(C) Western blotting and qRT-PCR analysis of HOXB6 and SOX9 expression in AAV-ctrl and AAV-Hoxb6 mice livers were performed.

Data are expressed as means ± SD, n = 3 independent experiments containing three replicates (A and B), n = 6 mice per group containing three replicates (C). ^∗p < 0.05 and ^∗∗p < 0.01 by two-tailed Student's t test.