Figure 2.

Increasing Ability of Cultured-Myogenic Cells of the CD34⁺ITGA7⁺ Fraction to Progress in the Differentiation Program

(A–F) (A–C) Representative density scatterplots showing the repartition of PAX7/MYOD (upper panel), MYOD/MYOG (lower panel) expression in cultured-myogenic cells. Debris and dead cells were excluded from the analysis. Gates were positioned based on FMO controls (Figures S2F and S2G). Graphs showing (D) the relative proportion of total PAX7+, MYOD+, and MYOG+ cells, and the repartition of (E) PAX7/MYOD and (F) MYOD/MYOG expression. Values are the mean ± SEM of three independent experiments (n = 3 mice/group). Statistical significance is represented relative to PAX7−MYOD+ cells (E) or MYOD+MYOG+ cells (F). Two-way ANOVA analysis, with ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.