Figure 3.

In Vivo Characterization of the Myogenic Populations from Birth to Adulthood

(A) Experimental timeline.

(B–G) (B) Strategy of analysis. Debris, doublets, and dead cells were excluded from the analysis. Representative density scatterplots showing the gates used to analyze myogenic cells in P7 mouse muscles. Joined gate PAX7+ or MYOD+ or MYOG+ cells was considered as the total myogenic cells (100%) referred to as TM. Representative density scatterplots showing, the repartition of (C) PAX7/MYOD and (D) MYOD/MYOG expression on TM (see also Figures S4A and S4B). Graphs showing (E) the proportion of total PAX7+, MYOD+, and MYOG+ cells, and the repartition of (F) PAX7/MYOD and (G) MYOD/MYOG expression on TM.

(H) Analysis of the myogenic cells was refined based on their cycling state, monitored by KI67 expression.

(I) Pie charts showing the distribution and the cycling state of the myogenic populations. Values represent the mean of data obtained from three to eight mice for each time point. Statistical significance is shown in Tables S5 and S6. Two-way ANOVA analysis, with ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, $ or ^∗∗∗∗p < 0.0001.