Figure 4.

Immunohistological Analysis of Myogenic Cells Location during Postnatal Growth

(A and B) Representative tibialis anterior (TA) muscle cross-sections immunostained for (A) PAX7 (green), MYOD (red), and laminin (yellow), (B) MYOG (green), MYOD (red), and laminin (yellow) (see also Figure S5). Nuclei were stained with DAPI. Scale bar, 20 μm.

(C–E) (C) Quantification of the number of PAX7+, MYOD+, and MYOG+ cells normalized per 100 fibers. Quantification of the proportion of sub-laminal and interstitial (D) MuSCs and (E) progenitors. Yellow and white magnification inlets indicate sub-laminal and interstitial PAX7+MYOD+ progenitors, respectively. Values are the mean ± SEM of data obtained from minimum three different mice for each time point. Two-way ANOVA analysis, with ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, $ or ^∗∗∗∗p < 0.0001 versus P0.