Figure 5.

Quiescent MuSC Pool Is Fully Established between P49 and P56

(A–C) (A) Representative TA muscle cross-sections immunostained for PAX7 (red), KI67 (green), and laminin (yellow). Nuclei were stained with DAPI. Scale bar, 20 μm. Quantification of (B) the total number of cycling (KI67+) and non-cycling (KI67−) PAX7+ cells per 100 fibers, and (C) the proportion of cycling and non-cycling PAX7+ cells determined on TA cross-sections immunostained as in (A).

(D) Representative density scatterplots showing the gates used to determine the proportion of cycling PAX7+ cells by flow cytometry.

(E) Proportion of cycling and non-cycling PAX7+ cells obtained by flow cytometry.

(F) Pie charts showing the repartition of MYOD+ and KI67+ cells among total PAX7+ cells. Statistical significance is shown in Table S7. Values are the mean ± SEM of data obtained from three to eight mice for each time point. Two-way ANOVA analysis, with $p < 0.0001 versus P0.