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. Author manuscript; available in PMC: 2021 Sep 10.
Published in final edited form as: J Med Chem. 2020 Aug 21;63(17):9977–9989. doi: 10.1021/acs.jmedchem.0c01111

Figure 6.

Figure 6.

(A) Compound 15 reduced the PRMT5 protein level in MCF-7 cells in a PRMT5-, E3 ligase VHL-, and proteasome-dependent manner. MCF-7 cells were treated with 5 μM of compound 15 for 7 d. During the last 24 h, the cells were co-treated with 100 μM VH-298, 2 μM MLN4924, 30 μM MG-132, or 30 μM EPZ015666. The protein levels of PRMT5 were detected by western blotting. (B) PRMT5 degradation induced by compound 15 was reversible. MCF-7 cells were treated with 5 μM of compound 15 for 6 d, and compound 15 was then washed out. The PRMT5 protein levels were detected by western blotting at 0, 12, 24, and 48 h post the washout.