KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| Clostridioides difficile R20291 | Stabler et al., 2009 | |
| Clostridioides difficile hsmR::CT | This study | |
| Clostridioides difficile hsmA::CT | This study | |
| Bacillus subtilis JH BS2 | Francis et al., 2013 | |
| Escherichia coli DH5α | Hanahan, 1983 | |
| Escherichia coli MG1655 | Blattner et al., 1997 | |
| Escherichia coli BL21(DE3) | Jeong et al., 2009 | |
| Staphylococcus aureus RN4220 | Kreiswirth et al., 1983 | |
| Staphylococcus aureus Newman | Duthie and Lorenz, 1952 | |
| Staphylococcus aureus ΔhrtB | Attia et al., 2010 | |
| Staphylococcus aureus ΔΔsod | Kehl-Fie et al., 2011 | |
| Staphylococcus aureus ΔkatA | Choby et al., 2018 | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Hemin from porcine | Sigma-Aldirch | Cat#51280–5G |
| Protoporphyrin IX | Sigma-Aldrich | Cat#P8293–1G |
| Paraquat dichloride hydrate | Sigma-Aldrich | Cat#36541–100MG |
| Vancomycin | Fisher Scientific | Cat#BP2958 |
| Metronidazole | Fisher Scientific | Cat#AC210340050 |
| Iron (II) sulfate | Sigma-Aldrich | Cat#215422–250 |
| RQ1 DNase | Thermo Fisher Scientific | Cat#M6106 |
| Ribolock RNase inhibitor | Promega | Cat#EO0381 |
| M-MLV reverse transcriptase | Thermo Fisher Scientific | Cat#M1705 |
| iQ SYBER green supermix | Biorad | Cat#1708880 |
| HisPur cobalt Resin | Thermo Fisher Scientific | Cat#25230 |
| HsmR | This paper | |
| HsmR H50A | This paper | |
| Cefoperazone | Sigma-Aldrich | Cat#C4292 |
| SYBR-safe | Invitrogen | Cat#S33102 |
| Dihydrorhodamine 123 | Invitrogen | Cat#DS2806 |
| Critical Commercial Assays | ||
| Thrombin Cleancleave kit | Sigma-Aldrich | Cat#RECOMT-1KT |
| RNeasy Mini Kit | Qiagen | Cat#74104 |
| Ribo-Zero rRNA removal kit | Illumina | Cat#2003715 |
| TruSeq RNA library prep kit v2 | Illumina | Cat#RS-122–2001 |
| Deposited Data | ||
| Raw RNA-sequence data | This Study | PRJNA576216 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J mice | The Jackson Laboratory | Cat #664 |
| Oligonucleotides | ||
| Primers for cloning and qRT-PCR: Table S1 | ||
| Recombinant DNA | ||
| Plasmids for complementation and expression: Table S2 | ||
| Software and Algorithms | ||
| TargeTronics algorithm | Targetrons | |
| PATRIC database | PATRIC | Version 3.5.43 |
| iTOL | iTOL | Version 4 |
| PubSEED | The SEED | Version 2.0 |
| GeneGraphics | Katlabs | |
| Prism | Graph Pad | Version 8.2 |
| CLC Genomics Workbench | Qiagen | Version 20.0.1 |
| HMMER | EMBL-EBI | Version 2.41.1 |
| MODELLER | University of California San Francisco | Version 9.24 |
| Canvas X16 | Canvas | Version 16 |