Figure 6.

Analysis of Somatic Mutations in Clonotype A

(A) Sequence alignment of the heavy and light chains of representative antibodies from clonotype A are shown. Residues of interest are highlighted in red with their positions indicated. EI, early intermediate.

(B) Residues of interest are shown on the crystal structure. HA surface is colored in gray, Fab heavy chain in pink, and light chain in cyan. The side chains of residues of interest are shown in stick representation. Since Gly100c does not have a side chain, the Cα of Gly100c is shown as a sphere.

(C) Binding curves of 54-1G07, 54-4H03, and (D) different 54-4H03 heavy-chain mutants paired with either the 54-4H03 light chain or the 54-1G07 light chain to H5 and H7 HAs as measured by meso scale discovery (MSD). Antibodies were in IgG format.