Extended Figure 10: Complex II is necessary for tumor growth.

a, Schematic representation of the ETC in complex II deficient 143B-Cytb-Δ cells expressing AOX. b, Western blot analysis of SDHA in 143B-Cytb-Δ non targeting (NT) and 143B-Cytb-Δ-SDHA_KOs cells. Tubulin was used as a loading control. Data representative of two independent experiments. c, Complex II driven oxygen consumption rate of permeabilized 143B-Cytb-Δ-NT-AOX and 143B-Cytb-Δ-SDHA_KO2-AOX cells. Piericidin A (1 μM) and Antimycin A (1 μM) were used to inhibit complex I and III respectively. SHAM (2 mM) was used to inhibit AOX activity (n=4 biologically independent experiments). d, 143B-Cytb-Δ-SDHA-KOs expressing GFP or AOX were grown in the presence or absence of methyl pyruvate and cell number was assessed after 72h (n=5 biologically independent experiments). e,f, Average tumor volume (e) and tumor mass (f) of xenografts from 143B-Cytb-Δ-NT-AOX and 143B-Cytb-Δ-SDHA_KO2-AOX cells (n=8 mice per group from two independent cohorts). Data represent mean ± s.e.m. (c-f). Statistical significance was determined using two-tailed t-tests (f) or 2-way ANOVA (d,e) with a Bonferroni test for multiple comparisons (*p < 0.05; **p < 0.01, exact P values in Source Data). For gel source data, see Supplemental Figure 6.