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. Author manuscript; available in PMC: 2021 Mar 2.
Published in final edited form as: Nature. 2020 Sep 2;585(7824):303–308. doi: 10.1038/s41586-020-2654-5

Figure 1: Phenobarbital binding sites.

Figure 1:

Panel a provides overview of atomic model of TMD viewed down channel axis from synaptic perspective; boxes highlight phenobarbital sites with ligand shown as spheres. Panel b shows the effect of mutation at the 15’ position of different subunits on fold potentiation of GABA activation by phenobarbital. The bars indicate mean ± SD, n = 7 (WT), 4 (αS270M), 5 (βN265M), 3 (γS280M) and 5 (double mutant) *, p < 0.01 ; ** p < 0.0001. “n=X” represents biologically independent patch clamp experiments with individual cells. Panels c and d show binding site details for phenobarbital at γ-β and α-β interfaces. H-bonds indicated with dashed line and distance.